shaker table rp4

shaker table rp4

Bacterial Conjugation Mediated by Plasmid RP4 - Journal of .Plasmid RP4 (IncP ) contains two transfer regions designated Tra1 and Tra2, both of ... concentration of 1 mM, and shaking was continued for 5 h. The cells were then harvested ... resistant derivative of RSF1010 (Table 1), which is mobilized.shaker table rp4,Components of the RP4 Conjugative Transfer Apparatus Form an .and shaking was continued for 4 to 5 h. Cells were . TABLE 1. Plasmids used for expression and purification of RP4 transfer gene products. Gene product.TraJ Protein of Plasmid RP4 Binds to a 19-Base Pair Invert .Jul 15, 2006 . Transfer of plasmid RP4 during bacterial conjuga- tion requires the .. IncP oriT Binding Protein. 11991. TABLE I. Purification of Trdprotein. Cultures (1 liter) of HBlOl . Shaking of cells was continued for 4 h. The cells were.

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  • shaker table rp4,

    Sequence Similarities between the RP4 Tra2 . - Semantic Scholar

    After shaking for 25 min . nucleotide sequence of the RP4 Tra2 region was determined .. TABLE I. Ribosome binding sites of predicted Tra2 genes trb gene.

  • transfer systems of RSF1010, RP4, and F. is a . - Semantic Scholar

    that of traG is related to TraG of RP4 and to VirD4 of the Ti plasmid T-DNA transfer system. Genetic .. (Table 1). The traA and traF genes were cloned into pET-14b as follows. A. 160-bp .. and all three were incubated with shaking for 30 min.

  • shaker table rp4,

    Plasmids Capable of Transfer and Chromosome . - CiteSeerX

    Plasmids used are listed in Table I . . for 24 h at 28 "C on a rotary shaker. . Table I. Plasmids. Incompatibility. Plasmid* group. Resistance markerst. RP4. R75 1.

  • RP4-mediated Conjugation in Acinetobacter . - Microbiology

    Sep 10, 1975 . A. calcoaceticus was detected, Transfer of RP4 between strains of A . calcoaceticus .. grown in 10 ml NB incubated for 18 h at 28 "C on a rotary shaker. . Table 2. Transfer of RP4 on Milliporefilters. Crosses were performed.

  • plasmid RP4 - PNAS

    plasmid RP4, the proteins TraI, TraJ, and Trai interact and assemble a ... fractions I-IlI (Table 1; 10, 7.5, and 5 Ag of protein, respectively); . Shaking of cells was.

  • Monitoring the conjugal transfer of plasmid RP4 in activated sludge .

    broad host-range plasmid RP4 to monitor the £ow . Table 1. Transfer of plasmid pMA119 (RP4::gfpmut3b) from P. putida SM1443 to different . with shaking.

  • Plasmids Capable of Transfer and Chromosome . - CiteSeerX

    Plasmids used are listed in Table I . . for 24 h at 28 "C on a rotary shaker. . Table I. Plasmids. Incompatibility. Plasmid* group. Resistance markerst. RP4. R75 1.

  • RP4-mediated Conjugation in Acinetobacter . - Microbiology

    Sep 10, 1975 . A. calcoaceticus was detected, Transfer of RP4 between strains of A . calcoaceticus .. grown in 10 ml NB incubated for 18 h at 28 "C on a rotary shaker. . Table 2. Transfer of RP4 on Milliporefilters. Crosses were performed.

  • Additional conjugation systems for inter-domain plasmid . - PeerJ

    Oct 4, 2017 . grown with shaking (200 rpm) at 37 °C in appropriate antibiotic ... predicted proteins from the RSF1010 and RP4/RK2 plasmids (Table 2).

  • Quantitative Techniques for Measuring Cleanroom Wipers with .

    RP4.3:2004 (2004) include particles (µg/cm2), extractables. (%), and ions (ppm). . specification of the wiper samples are listed in Table 1. For this research, three . the second method uses a more vigorous shaking of the wiper in a vessel.

  • On a vertex-minimal triangulation of $\mathbb {R} P^ 4$

    Dec 15, 2014 . other triangulation of RP4 on 16 vertices is known. Apart from the information .. In [15, Table 8.2], D.M. Mesner, at the time unaware of the pre- existence of W22 in the group .. Verlag Shaker, Aachen,. 1999. Dissertation.

  • Assessors Manual, Data Collection - Commercial - Department of .

    Sep 2, 2002 . Commercial - Table of Contents. Page 2 .. SHAKER Ltd. PO BOX 495 .. RP4 Porch, enclosed: A porch consisting of a wood roof over a.

  • PCR targeting system in Streptomyces coelicolor . - Streptomyces

    Table 1: Disruption cassettes containing different resistance markers with and without oriT: All .. Grow for 3-4 h at 30°C shaking at 200 rpm to an OD600 of ~ 0.4. 4. .. methylating E. coli ET12567 containing the RP4 derivative pUZ8002.

  • watson - Europe PMC

    Introduction of RP4 plasmid into Agrobacterium tumefaciens promotes the transfer on solid .. to maximize aeration. Recipient bacteria were grown shaking in . Table 1 shows the results of a typical conjugation experiment. The transfer of RP4.

  • RP1-13 - International Code Council

    The reasoning that proponents of RP4 used to support the elimination of any water test head pressure ... tests are commonly verified by the inspector “shaking the stack. .. In flood hazard areas as established by Table R301.2(1), plumbing.

  • shaker table rp4,

    resistance plasmids

    May 24, 2011 . harbouring RP4 plasmid was shown to result from evolution .. Table 1. Bacterial strains, plasmids and bacteriophages used in the study. strain.

  • the Burst - NASA Technical Reports Server (NTRS)

    LAD #13 Post-Thermal Resolution Prior to Re-Vibration ...... 45. LAD .. outlined in Table 1.2. Table. 1.2. Milestones of the BATSE-GRO. Project - 1977-1991. Event/Milestone. Date .. potentiometers. RP4, RP5, and RP6 to establish the.

  • Fight evolution with evolution: plasmiddependent phages with . - JYX

    contains a conjugative plasmid RP4 conferring resistance to several . 37°C. Shaking at 200 revolutions per minute (rpm) was .. ANOVA) are given in Table S1.

  • FEMA P-1050-2 - FEMA

    RP4-2.2 Replace ASCE/SEI 7-10 Section C21.2 with the following: . . RP5-2 Behavior of flexible diaphragm buildings subjected to Earthquake Ground shaking ...... 112. RP5-3 ... For Part 1 of the Provisions in Volume 1, its Table of.

  • A model for bacterial conjugal gene transfer on solid surfaces

    fluorescens MON787 acting as donor and recipient of plasmid RP4. ... 30‡C and with shaking at 175 rpm. .. rate were used as model constants (Table 1).

  • Science Journals — AAAS - Science Translational Medicine

    Jun 13, 2018 . (Table 1), and whole-genome sequencing confirmed that all planned mutations were .. then discarded, and the culture was moved to a shaker (250 rpm) for ... mobilized by broad-host-range RP4 conjugative machinery.

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