Bacterial Conjugation Mediated by Plasmid RP4 - Journal of .Plasmid RP4 (IncP ) contains two transfer regions designated Tra1 and Tra2, both of ... concentration of 1 mM, and shaking was continued for 5 h. The cells were then harvested ... resistant derivative of RSF1010 (Table 1), which is mobilized.shaker table rp4,Components of the RP4 Conjugative Transfer Apparatus Form an .and shaking was continued for 4 to 5 h. Cells were . TABLE 1. Plasmids used for expression and purification of RP4 transfer gene products. Gene product.TraJ Protein of Plasmid RP4 Binds to a 19-Base Pair Invert .Jul 15, 2006 . Transfer of plasmid RP4 during bacterial conjuga- tion requires the .. IncP oriT Binding Protein. 11991. TABLE I. Purification of Trdprotein. Cultures (1 liter) of HBlOl . Shaking of cells was continued for 4 h. The cells were.
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